Cubensis mycelium on agar

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Cubensis mycelium on agar

Anyone who really wants to dig into the hobby of growing mushrooms needs to learn how to deal with nutrient agar.

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Not only is it a way to experiment and learn to grow mushrooms more effectively, it allows you to save and propagate culturesallows you to create your own spawn and liquid cultures, and best of all… its fun! I want to show you how the best way to make agar petri dishes for growing mushrooms at home. Scroll to the bottom of the article for a video of the process! There are lots of different recipes for agar mix, including PDA potato dexrose agarMYA malt yeast agar and even DFA dog food agarbut they all consist of essentially the same idea- adding some sort of food source to agar to create a nutrient rich agar media for mushroom cultures.

The mix we use most is Malt Extract Agar. Agar might be a little hard to find locally. There are various sources online and some grocery stores will even carry blocks of Agar that can be ground up and used for your mix. Malt extract can usually be found a your local brew store. Nutritional yeast can usually be found at organic grocery stores.

Alternatively, you can buy premixed Malt Extract Agar powder that can just be added to water. Feel free to modify the recipe depending on how much agar you want. I usually make up ml agar mix in order to pour a sleeve of 25 mm x 15mm plates. Mix your dry ingredients in correct proportions with hot tap water. If the water is too cold it will start to set up right away and will be difficult to mix properly.

The agar mix will have to be sterilized at 15 psi for 45 minutes to kill all potential contaminants. I like to use a whisky bottle with a modified lid for sterilization and pouring. To do this, simply drill a hole in the lid and pull some through some pillow stuffing.

This filter will allow for sterile gas exchange while the agar cools off after sterilization. Cover the lid with tinfoil and place in the pressure cooker. A common problem when sterilizing agar in a pressure cooker is boil over. This caused when the water outside the bottle cools off faster than the agar inside the bottle, which forces the agar to boil over through the filter, making a mess in your cooker.

A way to remedy this is by adding lots of water to your pressure cooker so that the cool off rates will be similar. I usually place the bottle in the pressure cooker at an angle so that most of the agar will be at the same level as the surrounding water.

Boil over rarely ever occurs if using this method. Once the agar is sterilized, it will need to cool down sufficiently to be able to handle it, but be warm enough so that it remains in its liquid form.

This usually takes at least a couple hours. This is a good time to prepare the area where you will pour your plates and washing up for sterile work. Using agar requires an extremely high level of cleanliness and attention to potential contaminates. Any mold spore or other contaminant in the air, from your skin or fingernails or breath could ruin your project. If a contaminate land on your petri dish at any time, it will likely sprout into action, ruining your potential projects and cultures.

Wearing hands and arms, wearing freshly laundered clothes, wearing nitrile gloves and even surgical masks can greatly increase your chances of success.

These tools will provide a clean area to pour your plates, and without using either of them, it is almost certain that your plates will contaminate.Did you use this instructable in your classroom? Add a Teacher Note to share how you incorporated it into your lesson. To begin growing your own mushrooms you will need the following for the PF tek: - Pressure cooker Check thrift stores such as savers and goodwill, also browse your local craigslist.

Be sure to use wide mouthed tapered jars! Spore syringes are the easiest way to get started growing mushrooms with this technique. They can be ordered online from a variety of sources. To prepare your jars, you will need a hammer and nail. Remove the jar lid and lay the flat lid rubber side up on a table.

Take a nail and make 2 - 4 holes evenly spaced around the edge. The sharp edge around the hole can tear the foil in the next few steps. The substrate is what the fungus will feed off of. It will consist of brown rice flour, vermiculite and water. First, take the dry ingredients and mix them in a bowl. After they are well mixed add the water. I have heard of adding 'worm castings' earthworm poop to substrate to give better flushes of mushrooms.

Once your substrate is well mixed and moist, begin filling your prepared jars with the substrate. Don't pack the mixture down, just loosely fill the jar. I just filled my jars up to the threading for the lid. Fill the rest of the jar with dry vermiculite. This is to make a barrier between the substrate and contaminants in the air.

Now close up all your jars, with the rubberside facing upwards upside down from how they were designed Place a square of foil over the lid covering the holes and sealing the jar from contaminants in the air.

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Crumple it down nice and tight, forming it around the jar lid. Put about 3 inches of water in your pressure cooker and place as many jars as you can, stacking them if you have to.

I had no problem putting jars directly on the bottom of the cooker, but I have seen people put old canning jar rings in the bottom to help prevent jars from cracking. READ your pressure cooker's manual if you have it! It can be a dangerous tool if improperly used.

Close up your cooker and put it on a stove. I used a BBQ side stove to keep heat out of the house.Log in or Sign up. Joined: Jul 8, Messages: 1, Location: canada. I was out in my garage where I put any contaminated jars until I'm ready to do a big clean up and i discovered this jar of Cope Cyan Hawaian Ive been having a hell of a time cleaning up a dirty wild print over the last four months or so just too stubborn to give up i don't know how many agar plates I've been through but it's a lot.

The bacterial rods must be embedded into the spores well once I seen this jar and how the mycelium had developed and was fighting for a chance the never give up kicked in again The jar was heavily infected with Trich when I put it in the garage,the spores would germinate and a tiny growth of mycelium would fan out from a single finch seed then be overcome with Trich I will be updating with pics on the first transfer Notice how the mycelium has roped out trying to get a foothold on a non infected nutrient source.

Joined: May 29, Messages: 3, Location: the armpit of america. Major MycSep 20, As a general rule I always make my agar plates less nutrtious same with LC when I used to do them thanks for the tip though. Thanks for both of your advice guys much appreciated I've cleaned up more than a fair share of dirty cultures and this one is going to be particulary difficult do to the extreme nature of the contamination As I stated I usually alway cut my ME for agar plates and LC's the rare times I do use LC"s and I never add dextrose,this appies to any agar plates I make not just for cleaning contaminated cultures.

Professor PinHeadSep 21, Joined: May 27, Messages: 1, Actually, you want to contams to grow so you can see them and transfer away.

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You don't want to inhibit the growth of mold or other contams, that just hides the contam for later. Also, mold is fungus. It's not something else. Can't believe I'm arguing over why a lesser nutritious agar formula is beneficial to the cleaning of cultures and cloning directly to agar. THAT'S the point of a lesser nutritios agar medium when it comes to cleaning myce. I use a flowhood so I prefer NOT to let the infection germinate.

That ups the risk of spreading the contaminates' spore load in my lab. If you let the culture grow too long, the invader spores WILL catch up to the leading edge of the culture and infect the entire culture. I liked your point about NOT looking for chemicals to combat mold, yet another living organism such as a parasite, you posted in another thread. Good point.

cubensis mycelium on agar

So, it's your opinion that cubensis mycelium grows faster than Trichoderma mycelium on less-nutritious media? If that were true, spawning to coir would work perfectly every time and would never contaminate. But I'll keep an open mind on the matter until I see the experiment you use to prove it. Mold is fungus, it grows the same as other fungus. It's a side effect of the Whorf-Sapir hypothesis that we want to consider them as being different things when they aren't. On a 2 dimensional surface such as agar yes.

A bulk substrate, grain medium, or LC is three dimensional thus invader spores can germinate in any which direction they desire to cause an infection. Look this isn't my opinion. I leaned this from RR a couple years ago. Ever since I realized what he was saying, been practicing cleaning myce. One look at a contam. Not saying you're incorrect. I just explained further, the scientific applications and principals behind making an agar medium less nutritious for the purpose of cleaning myce.

I'm sorry if I'v offended you. There's just more to it then simply, a lesser nutritious agar medium favors myce. Molds and bacteria will grow just splendidly on a substrate with little nutrition.Mountain Mushroom Farm. Wood Fired Steam Boiler. Rye Grass Seed Preparation. Grain to Grain Transfers. Casing Layer Preparation. Straw Pasteurization. Laundry Baskets-Straw Logs.

Horse Manure Substrate. Agar-Petri Dishes. Master Culture Slants. The starting point for mushroom mycelium is the spore. Any contamination they encounter in our artificial environment at this point is likely fatal to them. There seems to be a mindset among amateur mushroom growers that using agar is hard. Careful attention to sterile procedure is a must. The secret to how a glovebox works is still air. Many growers waste endless time and energy trying to Lysol and bleach the inside of their gloveboxes, when a simple washing with soap and water would suffice.

The reality is that wind currents are what drives contaminants into our sterile media. If we can stop the air from moving, we can safely open a Petri dish for a short period of time with reduced chances of contaminant spores entering. Always transfer a tiny piece of mushroom mycelium and move it away from the contamination to a new dish.

The smaller the piece of mycelium you cut, the fewer contaminant spores are going to be on it, and therefore the greater your chance of success.

Simply continue making transfers as necessary until you have clean, pure mushroom mycelium. Never try to transfer contamination away from your mushroom mycelium.

This chapter of Let's Grow Mushrooms demonstrates sterile procedure and one method for preparing the agar and pouring Petri dishes. The work is demonstrated in front of a laminar flow hood to make filming easier.People often struggle with these common mushroom cultivation mistakes, when they try to grow for the first time.

When you are first starting off your mushroom cultivation journey, be sure to look out for these. Here are some mistakes that we made when we first started mushroom growing. Contamination is the lead cause of failure when growing mushroom. Incorrect sterilization is the lead cause of contamination. You must treat all equipment, work surfaces, containers and gloves with alcohol before any work commences.

The working environment is just as important. It is vital to keep a clean, tidy and sterile work environment, in order to maximise the chances of success.

cubensis mycelium on agar

First-time mushroom growers are always eager to get to the finished product. Mushroom Growing can be a meticulous and thorough process that you should not rush. Incomplete preparations or not following and sticking to one chosen technique will lead to a higher risk of contamination.

Trying to rush each stage will also lead to a high failure rate. Always allow your substrate to completely cool before inoculation.

Growing Mushrooms: PF Tek

Be sure that Mycelium has fully colonized your substrate before starting the fruiting stage. There is often a temptation to cut corners in order to save costs. There is no justification for this when growing mushrooms. The cost of production is so low, to begin with, that it is worth investing fully into the right equipment from the start to increase your success. The wrong environment will quickly lead to failures.

It is very important to always check the requirements for each different strain of mushrooms that you grow.

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Mushrooms grow in a multitude of habitats around the world, so each one requires separate attention. Most critical aspects are:. Thermogenesis happens when the mycelium is decomposing organic matter.

Like compost piles, the mushroom substrate will start generating its own heat. There is a possibility that the temperature in your substrate will become higher than the air temperature in the room.

If your fruiting room is running at the optimum temperature, then your substrate could rise above it and cook the Mycelium. This could mean that your mycelium will get cooked and die. There are some mistakes that can only be avoided with experience that you gain by cultivating your own mushrooms.

Here are some of the common things that will dramatically increase your success rate:. It is important that you start practicing proper sterile methods early in your mushroom cultivation journey.

Most contamination issues can be avoided by proper technique and good equipment.

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Make sure that your environmental conditions are proper for the type of mushroom you are growing. Humidity, air exchange, light, and temperature are all important factors to keep a constant watch on.I've decided to put this guide together because there are numerous old threads and outdated information floating around regarding the cultivation of the sclerotia producing Psilocybes.

There are however some really great threads too, like the Easier than Cubes!

cubensis mycelium on agar

I have worked mainly with these four stone producers for the last year. Section Mexicanae is my absolute favorite from the Psilocybe genus. As you probably already know these species have the ability to produce sclerotia stones invitro and fruit bodies as well.

Most people cultivate them for stones only but hopefully after reading this thread they'll change their mind and give it a go at fruiting 'em too. They aren't much harder to fruit than Psilocybe cubensis just require a thin casing layer and some extra TLC. Let's get started! Here's a short version of those three.

Steam sterilize for 90 minutes or PC for 60 minutes 15psi. Psilocybe tampanensis sclerotia forming on BRF substrate. Shake to distribute the liquid evenly and let sit for two hours.

Agar101 - Beginners Agar Kit

Then PC it for 60 minutes 15psi. Psilocybe galindoi sclerotia on Rye Grass Seed. I hate WBS because it's messy and pretty difficult to clean the millet away from the sclerotia. Use it however if for some reason rye or RGS is not available.

You can also prepare it at the same way like the rye berries below. Psilocybe tampanensis colonizing on Wild Bird Seed. This is basically identical to RR's Rye Grain Preparation with the addition of coffee to the soak water. I start it by brewing a full pot 2 quarts of strong coffee. Add a couple of teaspoons of gypsum and stir. Use a cup of rye for each quart jar.Psilocybe cubensis is a species of psychedelic mushroom whose principle active compounds are psilocybin and psilocin. Their caps are planar when fully mature, and their gills are adnate horizontally attached to the stem to adnexed slightly indented at the attachment point depending on the variety.

The mycelia are like microscopic straws that look similar in appearance to foam or hoarfrost, very similar to the findings of Moses in Exodus The gills are closely spaced and contain purple spores that can germinate once they are in a suitable substrate. Psilocybe cubensis is a coprophilic fungus a dung-loving species that often colonizes the dung of large herbivores, most notably cows and other grazing mammals such as goats.

cubensis mycelium on agar

It prefers humid grasslands and has been found in tropical and subtropical environments. In the US, it is sometimes found growing wild in the South, generally below the 35th parallel. It has been found in modern times in the highlands and river valleys of ArgentinaColombiaEcuadorPeru and Venezuela in South America. It has also been found throughout ThailandCambodiaIndiaand Australia. The reason cubensis grows commonly on the dung of these animals is because they have no or very little stomach acid.

The cow eats feed with mushroom spores on it, and the spores germinate in the cow's moist, warm stomach. No, cubensis is not found under cow patties, and you should not consume anything you find under cow pies.

Unless you're a badass mycologist, and know what sclerotia is, what species can form sclerotia, and are in the part of Mexico where it is commonly found.

Working With Mushroom Cultures on Agar

Cubensis can be found within a few hundred miles of the Gulf Coast reliably, especially in fall and spring, all the way from Galveston, TX to Miami, FL. Note though that after 11 years of diligent wild hunting, I've only found cubensis in TN twice.

If you hunt wild shrooms, make sure you are an expert at identifying mushrooms. What's the worst that could happen if you mis-identify? Your liver will shut down in a day or two, and you'll die of systemic ammonia toxicity and jaundice within a week or so.

Yeah, you want to be careful. I also found it in New Jersey. Spawn run. Since cubensis are a dung-loving speciesthey will grow exceptionally well on horse and cow manurethey will also produce well on many other substrates such as coconut coirstraw and whole grains. The PF tekarguably the most popular growing technique for cubensis, allows a cultivator to get many flushes out of just vermiculite and brown rice.

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Another good method is to soak your cakes in sterile water over night then roll in a substrate like vermiculite or just roll it in your old coffee grounds. Works amazingly! Although cubensis will benefit from a casing layer and are able to conquer a relatively deep one, they will fruit well directly from the substrate alone.

Agar agar for shrooms / mushrooms magic, recipe part.1

In addition, the higher temps tend to stimulate thermophic molds and bacteria. Cubensis are extremely resilient to various temperatures when they are in their fruiting stage, but like many other species, will produce higher quality, denser fruit bodies when lower temperature is maintained. Fruit bodies will grow in less than saturated air, but primordia will not form well.

Cubensis will be far more resilient to changes in humidity if a casing layer is applied. Cubensis is the most popular psychoactive mushroom among hobby cultivators due to the the ease with which it can be grown. The PF teka famous mushroom cultivation method, was originally designed with this species in mind. Cubensis produce a very rhizomorphic mycelium and will fruit well on a wide variety of substrates without a casing layer.

Its major psychoactive compounds are:. Psilocin and psilocybin are substances isolated by Albert Hofmann in in a related and less potent species, P. All four compounds are presumed hallucinogenicthough it is suspected that baeocystin and norbaeocystin are less psychoactive than psilocybin and psilocin. Individual brain chemistry and psychological predisposition play a significant role in determining appropriate doses.

For a modest psychedelic effect, a minimum of one gram of dried cubensis mushrooms is ingested orally. For most people, 3.


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